Cryo
The outbreak of a novel coronavirus (2019-nCoV) represents a pandemic threat that has been declared a public health emergency of international concern. The CoV spike (S) glycoprotein is a key target for vaccines, therapeutic antibodies, and diagnostics. To facilitate medical countermeasure development, we determined a 3.5-angstrom-resolution cryo-electron microscopy structure of the 2019-nCoV S trimer in the prefusion conformation. The predominant state of the trimer has one of the three receptor-binding domains (RBDs) rotated up in a receptor-accessible conformation. We also provide biophysical and structural evidence that the 2019-nCoV S protein binds angiotensin-converting enzyme 2 (ACE2) with higher affinity than does severe acute respiratory syndrome (SARS)-CoV S. Additionally, we tested several published SARS-CoV RBD-specific monoclonal antibodies and found that they do not have appreciable binding to 2019-nCoV S, suggesting that antibody cross-reactivity may be limited between the two RBDs. The structure of 2019-nCoV S should enable the rapid development and evaluation of medical countermeasures to address the ongoing public health crisis.
Cryo
The National Cancer Institute launched the National Cryo-EM Facility in May 2017 as a federally funded pilot effort to meet the needs of cancer researchers in United States academic laboratories who do not have adequate access to cryo-electron microscopy instruments.
The Cryo-EM core facility at Montana State University is located in the Chemistry and Biochemistry department, and access and instrument time is available to all with advanced scheduling. The current capabilities of the facility include TEM imaging, cryo-grid preparation and screening, single particle analysis, and cryo-electron tomography.
Funding to establish the cryo-EM core facility was provided by the National Science Foundation, the Murdock Charitable Trust, and MSU's Office of Research, Economic Development, and Graduate Education
Titan Krios is a state-of-the-art sophisticated high-voltage (300kV) transmission electron microscope. It has a superb electronic optical system and a capability to do fast high-throughput data collection. Titan Krios is equipped with the latest Gatan Image Filter (GIF) and Gatan K2 Summit direct electron detector. This combination allows better electron signal to be recorded. This configuration is currently the ideal setup for high-resolution structure visualization, using single-particle electron cryomicroscopy and electron tomography.
Both microscopes are equipped with a Volta Phase Plate to enable visualization of smaller macromolecular complexes. The facility has additional equipment for sample preparation and storage, including two FEI Vitrobot Mark IV, a glow discharge unit, an auto-filled 55-cylinder cryo-grid storage system etc.
Additionally, the Molecular Electron Microscopy Suite (MEMS) at Harvard Medical School is a separate user resource currently available to qualified researchers. This user facility offers training and supervision in negative-stain and cryo-transmission electron microscopy. Equipment includes three transmission electron microscopes, two cryo plungers, and sample preparation areas.
The Electron Microscopy Resource Lab (EMRL) at the Perelman School of Medicine, University of Pennsylvania, is a training and service facility dedicated to providing both conventional transmission electron microscopy (TEM) of cells and tissues and state of the art cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) for structural investigation of macromolecules and cells. The core facility offers services to University of Pennsylvania research groups and external academic research groups in the greater Philadelphia area.
The Cryo-EM Facility at Iowa State University provides training, education and support in all aspects of cryo-EM sample preparation, data collection, and single-particle data analysis and structure determination. The facility continues to test and improve high resolution validation, but training and services for sample preparation and screening for Cryo-EM grids are available. The establishment of the Cryo-EM Facility was supported by a grant from the Roy J. Carver Charitable Trust.
The facility houses a Thermo Fisher Scientific 200 kV Glacios microscope equipped with a K3 Direct Electron Detector (DED) for high-resolution cryo-EM and a Vitrobot for sample preparation. The facility has dedicated 250-TB local storage and 28 GPUs (seven nodes) for computation. Policies, rates and booking details can be found on this website.
The Cryo-Electron Microscopy (CRYOEM) core provides the NIDDK scientific community with equipment and training in preparing and imaging negative stain and cryo-EM grids. The facility has three transmission electron microscopes, Glacios (K3 camera), Tecnai TF20 (K2 camera) and a T12, all optimized for negative stain, cryo-EM Single Particle and Tomography imaging. The Glacio and TF20 are in Building 8, room B2A20-24 and the T12 is in Building 50, room B406 . The facility also has two EM grid plungers, a carbon evaporator, a glow discharge machine, and a rapid freezing device (Chameleon) located in building 8, room 1A18.
The Stanford cEMc enables macromolecular structure determination, mechanistic investigations, and drug discovery through cutting-edge cryo-electron microscopy (cryoEM) for the Stanford community and beyond.
The Columbia University Cryo-Electron Microscopy Center (CEC) provides researchers with training and access to the advanced instrumentation, data collection capacity, and processing support required to incorporate cryo-electron microscopy into their studies. The CEC supports instruments at the Medical Center, Zuckerman Institute, and New York Structural Biology Center campuses, but operates as a single core facility, allowing users to use instruments across locations.
Whole Body Cryotherapy is not just a fancy ice bath; it works through a totally different mechanism. Ice simply penetrates the skin, cooling the tissues and muscles and causing blood vessels to constrict. It can often be uncomfortable, and prolonged use can actually freeze and damage the muscle. The cold sensation of cryotherapy does not penetrate the skin, but the body still responds as if it is freezing, and immediately activates a recovery response.
Cryo Products specialises in the field of cryogenic applications. Evidently we provide high-quality, dedicated service based on innovation, a solution and a customer oriented approach; plus the creation and implementation of new ideas. Additionally this combination makes Cryo Products a highly dependable partner in the cryogenics market.
[00:01:32] My speciality is cryo electron microscope. And we look at three dimensional structures of proteins and nuclear protein complexes. So these are what I refer to as bio nano machines. These are these tiny molecular machines that carry out processes in cells to sustain life.
The program is broadening access to high-resolution cryoelectron microscopy (cryoEM) and tomography (cryoET) for biomedical researchers by creating national service centers, and cultivating a skilled workforce through the development and implementation of cryoEM training material. CryoEM and cryoET enable high-resolution, three-dimensional data collection on samples that could not be used with other techniques in the past, such as samples containing mixtures of different biological structures or intact cells. The three National cryoEM Service Centers offer usage of state-of-the-art equipment, technical support, and cross-training for the production and analysis of high-resolution data. These offerings are available at no charge for non-profit use, eliminating the high cost barrier usually associated with cryoEM. The NIH recently funded four centers to make up the National Network for CryoET. This Network will provide the biomedical research community access to advanced instrumentation for cryoET, cryoET specimen preparation, and collection of high-resolution cryoET data as well as cross-training in cryoET methods. Check back for updates on this exciting new resource!
Open access instructional material on cryoEM for those with or without a structural biology background is already available through the CryoEM Curriculum Development sites. Further developments are expected soon.
Did you miss one of the free monthly webinars on cryoEM current practices and strategies hosted by the National Centers for Cryoelectron Microscopy? Catch up before the next one by watching recordings!
Our complete range of products intended for human application allows the freezing at very low temperature of any biological sample whatever its physical state. Our unique high-security concept for long-term cryopreservation guarantees unsurpassed security and storage quality.
The Sauer Structural Biology Laboratory (SSBL) is a state-of-the-art cryo-EM facility. The SSBL offers assisted and unassisted use of our high-end TEMs and ancillary sample preparation equipment, training, and collaborations.
The Purdue Cryo-EM Facility is a member of the Purdue EM Consortium and a designated Indiana CTSI Core Facility. Our facility is available to Purdue, other academic institutes, and industry users. It provides state-of-the-art instruments and expertise for high resolution structure determination of viruses, larger macromolecular complexes, nano-particles, as well as tomographic visualization of virus-cell interactions by cryo-electron microscopy (cryo-EM) and three-dimensional (3-D) image reconstruction. 041b061a72