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Jesus Zykov
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The Mirum Download ((EXCLUSIVE)) Requirements



If you'd like to prescribe LIVMARLI for your patient, download the Patient Enrollment Form. Once you have filled out the form and/or have your patient consent for MAP services, fax it to MAP at 1-855-282-4884.




The Mirum download requirements


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We wanted the campaign to be as accessible as possible with minimal barrier to entry. With modern mobile apps there is always this barrier when we require people to download the apps first. At Mirum, we understand these limitations and found a framework that allowed us to bring the full potential of AR applications onto the modern web without the need for native mobile apps. We utilized the technology offered by 8th Wall to create a web AR version that is accessible on any device capable of using a web browser and camera.


Based on DNA analyses, Simulium sp. is proven to show dimorphism in males and is herein described as a new species, Simulium mirum Takaoka, Sofian-Azirun & Low. This is the first report of such a novel species among the family Simuliidae.


Larva of Simulium mirum n. sp. (a) Head capsule showing postgenal cleft (ventral view). b Mandible. c Hypostoma. Scale-bars: 0.05 mm for (a); 0.02 mm for (b) and (c)


To comply with the regulations set out in article 8.5 of the amended 2012 version of the International Code of Zoological Nomenclature (ICZN), details of the new species have been submitted to ZooBank. The Life Science Identifier (LSID) of the article is urn:lsid:zoobank.org:pub:27FDFE51-721B-488C-BAAF-010822CDD626. The LSID for the new name Simulium mirum is urn:lsid:zoobank.org:act:E8B9D049-317B-4F8F-9235-53222BC3A172.


The cause of the dimorphism shown in the males of S. mirum n. sp. is unknown. Unlike the case of S. gonzalezi, there seems to be the least possibility, if any, that it has been caused by the intersexuality. It is because the heads of both form A and form B males are holoptic (a typical expression of the male heads, i.e., the left and right eyes medially contiguous, not separated by the frons) (Fig. 1b, c), though the numbers of the enlarged upper-eye facets are different between form A and form B, thus having nothing to do with the expression of the feminine head morphology. In general, the abnormalities resulted from the intersexuality are mostly expressed longitudinally (e.g., the head feminine, the thorax and abdomen masculine, or vice versa), or asymmetrically in paired features (e.g., the left side of the body feminine, the right side masculine, or vice versa), or intermediate (e.g., two intersex specimens in the type-material).


Dimorphism in the males of S. mirum n. sp. may have originated before this new species had spread in Sabah and Sarawak because it was observed in at least two locations (Timpohon and Mesilau) in Sabah and three locations (Bakalalan, Bario and Pueh) in Sarawak (HT, unpublished data). Further studies are needed to determine the frequency of occurrence of each male form in each population and the underlying chromosomal and genetic mechanisms of this phenomenon.


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Requiem and Kyrie (Solo Quartet, Chorus)Dies irae Dies irae (Chorus) Tuba mirum (Chorus) Mors stupebit (Bass) Liber scriptus (Mezzo-Soprano, Chorus) Quid sum miser (Soprano, Mezzo-Soprano, Tenor) Rex tremendae (Solo Quartet, Chorus) Recordare (Soprano, Mezzo-Soprano) Ingemisco (Tenor) Confutatis (Bass, Chorus) Lacrimosa (Solo Quartet, Chorus)Offertorium (Solo Quartet)Sanctus (Double Chorus)Agnus Dei (Soprano, Mezzo-Soprano, Chorus)Lux aeterna (Mezzo-Soprano, Tenor, Bass)Libera me (Soprano, Chorus)


Figure 1. Chromosome Atlas of Spiroplasma mirum. Moving inside, each concentric circle represents a different genomic feature. The outermost circle shows predicted protein-coding sequences on both strands, colored by functional categories according to COG classification. The second circle displays specific genes of S. mirum genome compared with the other. The third circle illustrates tRNA genes on plus strand (blue) and minus strand (red), and ribosomal RNA genes (green). The fourth circle presents IS elements. The fifth circle shows mean centered G + C content (red-above mean, blue-below mean). The sixth circle (innermost) represents GC skew (G - C)/(G + C) calculated using a 1 kb window.


Figure 3. (A) Comparison of COG distribution of Spiroplasma eriocheiris and Spiroplasma mirum. (B,C) COG classification of S. mirum and S. eriocheiris, respectively.


Figure 4. Schematic representation of metabolic pathways of Spiroplasma eriocheiris (left side) and Spiroplasma mirum (right side). Shown are transporters and the main elements of metabolic pathways, deduced from the set of genes with predicted functions. Black arrows: pathways found in both S. eriocheiris and S. mirum genomes. Blue arrows: S. eriocheiris special in Mollicutes. Red arrows: pathways found in the S. eriocheiris genome but not in the S. mirum genome. : absent enzyme in pathways. Transporters are colored according to their substrates: blue, cations; green, anions and amino acids; rad, carbohydrates; pink, multidrug, and metabolic product efflux. Arrows indicate the direction of substrate transport. In some cases, we cannot identify all the components of these transporters.


Figure 5. (A) Comparison of the gene order between Spiroplasma eriocheiris and Spiroplasma mirum. (B) Protein synteny plot of S. eriocheiris and S. mirum.


Citation: Liu P, Li Y, Ye Y, Chen J, Li R, Zhang Q, Li Y, Wang W, Meng Q, Ou J, Yang Z, Sun W and Gu W (2022) The genome and antigen proteome analysis of Spiroplasma mirum. Front. Microbiol. 13:996938. doi: 10.3389/fmicb.2022.996938


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